Fluorescent confocal image of A549 cell stained with ALDH2 Antibody. A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ALDH2 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). ALDH2 immunoreactivity is localized to Mitochondrion significantly.

ALDH2 Antibody western blot of A549,HepG2,NCI-H292 cell line,mouse liver,lung and rat liver tissue lysates (35 ug/lane). The ALDH2 antibody detected the ALDH2 protein (arrow).