Confocal image of mixed rat neuron-glial cultures stained with our rabbit polyclonal antibody to ACIII (red) and our mouse monoclonal antibody to aII-spectrin (MCA-3D7 green). Note the strong and clean staining of neuronal cilia. Since aII-spectrin is specific for neurons in the CNS, the glial cells in this culture are not recognized by this antibody. The aII-spectrin antibody is also an excellent marker of neuronal plasma membranes.
Western blots of rat olfactory epithelium (OE) and frontal cortex (FC). The antibody stains bands at about 200kDa in olfactory epithelium which is rich in cilia. Fewer cilia are found in frontal cortex, and the protein is less heavily glycosylated, and a less prominent band is seen at about 160kDa. Data generated in the laboratory of Matt Sarkisian in the University of Florida.