Western blot with extracellular domains (ecd) of different related human receptors (50ng per lane) reveals that anti-Activin Receptor IIB, pAb (IG-510) (dilution 1:5000) specifically recoginzes ACTR-IIB under non reducing (-[Character df]ME, left panel) and reducing (+[Character df]ME; right panel) conditions, but none of the other receptor types tested.
ELISA detection using anti-Activin Receptor IIB, pAb (IG-510) . Wells were coated with 100ul of ACTR-IIB extracellular domain (ecd) at 2ug/ml. For detection different dilutions of anti-Activin Receptor IIB, pAb (IG-510) stock solution (500ug/ml) were applied, followed by an ALP-conjugated goat-anti-rabbit antibody.