Anti-ZO1 tight junction protein antibody
| Name | Anti-ZO1 tight junction protein antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab59720 |
| Prices | $391.00 |
| Sizes | 50 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF FC WB |
| Species Reactivities | Mouse, Chicken, Dog, Human, Pig |
| Antigen | Corresponding to amino acids 432-1150 of Human ZO1 tight junction protein |
| Description | Rabbit Polyclonal |
| Gene | TJP1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab59720 staining ZO1 in Caco-2 cells treated with (±)-palmitoylcarnitine chloride (ab141122), by ICC/IF. Membranar ZO1 expression loss correlates with increased concentration of (±)-palmitoylcarnitine chloride, as described in literature.The cells were incubated at 37°C for 1 hour in media containing different concentrations of ab141122 ((±)-palmitoylcarnitine chloride) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab59720 (0.5 μg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
ICC/IF image of ab59720 stained MCF-7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab59720, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunofluorescence analysis of HUVEC cells, staining ZO1 tight junction protein with ab59720. Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 1% BSA for 30 minutes at 25°C. Samples were incubated with primary antibody (1/200 in diluent) for 16 hours at 4°C. An AlexaFluor®488-conjugated goat anti-rabbit polyclonal IgG (1/500) was used as the secondary antibody. See Abreview
Immunofluorescence analysis of murine peripheral retinal pigmented epithelium (RPE) cells, staining ZO1 tight junction protein with ab59720. Cells were fixed with 4% paraformaldehyde for 10 minutes at room temperature. Cells were blocked in 10% BSA and 0.5% Triton-X 100 for 2 hours at room temperature before being incubated with primary antibody (1/100) in blocking solution overnight at room temperature. An AlexaFluor®-conjugated anti-rabbit IgG was used as the secondary antibody.
Product References
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