Lane 1 : Anti-Wnt10a antibody (ab106522) at 1 µg/mlLane 2 : Anti-Wnt10a antibody (ab106522) at 2 µg/mlLane 1 : RAW264.7 cell lysateLane 2 : RAW264.7 cell lysateLysates/proteins at 15 µg per lane.
ab106522, at 10 µg/ml, staining Wnt10a in Human skeletal muscle tissue by Immunohistochemistry.
Immunohistochemical analysis of rat bladder tissue, staining Wnt10a with ab106522.Tissue was fixed with formalin and blocked with 5000 µg/ml BSA for 30 minutes at 22°C; antigen retrieval was by heat mediation in citrate buffer (pH 6). Samples were incubated with primary antibody (1/100 in BSA) for 1 hour at 22°C. An AlexaFluor®555-conjugated goat anti-rabbit polyclonal IgG (1/400) was used as the secondary antibody. See Abreview
Immunofluorescence of Wnt10a in Human Skeletal Muscle cells using ab106522 at 20 ug/ml.