![Lanes 1 & 4 : Anti-VRK1 antibody [5D1] - N-terminal (ab171933) at 1/100 dilutionLanes 2 & 5 : Anti-VRK1 antibody [5D1] - N-terminal (ab171933) at 1/500 dilutionLanes 3 & 6 : Anti-VRK1 antibody [5D1] - N-terminal (ab171933) at 1/1000 dilutionLane 1 : HeLa cell extract (5x10e4 cells)Lane 2 : HeLa cell extract (5x10e4 cells)Lane 3 : HeLa cell extract (5x10e4 cells)Lane 4 : U2OS cell extract (5x10e4 cells)Lane 5 : U2OS cell extract (5x10e4 cells)Lane 6 : U2OS cell extract (5x10e4 cells)SecondaryAlexa488 goat anti-mouse IgGdeveloped using the ECL technique](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/20574_ab171933-196989-171933.JPG)
Lanes 1 & 4 : Anti-VRK1 antibody [5D1] - N-terminal (ab171933) at 1/100 dilutionLanes 2 & 5 : Anti-VRK1 antibody [5D1] - N-terminal (ab171933) at 1/500 dilutionLanes 3 & 6 : Anti-VRK1 antibody [5D1] - N-terminal (ab171933) at 1/1000 dilutionLane 1 : HeLa cell extract (5x10e4 cells)Lane 2 : HeLa cell extract (5x10e4 cells)Lane 3 : HeLa cell extract (5x10e4 cells)Lane 4 : U2OS cell extract (5x10e4 cells)Lane 5 : U2OS cell extract (5x10e4 cells)Lane 6 : U2OS cell extract (5x10e4 cells)SecondaryAlexa488 goat anti-mouse IgGdeveloped using the ECL technique
![All lanes : Anti-VRK1 antibody [5D1] - N-terminal (ab171933) at 1/500 dilutionLane 1 : Extracts from TIG1 (5x10e4) cells: Luciferase RNAi (control)Lane 2 : Extracts from TIG1 (5x10e4) cells: VRK1-1 RNAiLane 3 : Extracts from TIG1 (5x10e4) cells: VRK1-2 RNAideveloped using the ECL technique](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/20575_ab171933-196991-1719333.JPG)
All lanes : Anti-VRK1 antibody [5D1] - N-terminal (ab171933) at 1/500 dilutionLane 1 : Extracts from TIG1 (5x10e4) cells: Luciferase RNAi (control)Lane 2 : Extracts from TIG1 (5x10e4) cells: VRK1-1 RNAiLane 3 : Extracts from TIG1 (5x10e4) cells: VRK1-2 RNAideveloped using the ECL technique
![All lanes : Anti-VRK1 antibody [5D1] - N-terminal (ab171933) at 1/500 dilutionLane 1 : Extracts from HeLa (5x10e4) cells: Luciferase RNAi (control)Lane 2 : Extracts from HeLa (5x10e4) cells: VRK1-1 RNAiLane 3 : Extracts from HeLa (5x10e4) cells: VRK1-2 RNAideveloped using the ECL technique](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/20576_ab171933-196990-1719332.JPG)
All lanes : Anti-VRK1 antibody [5D1] - N-terminal (ab171933) at 1/500 dilutionLane 1 : Extracts from HeLa (5x10e4) cells: Luciferase RNAi (control)Lane 2 : Extracts from HeLa (5x10e4) cells: VRK1-1 RNAiLane 3 : Extracts from HeLa (5x10e4) cells: VRK1-2 RNAideveloped using the ECL technique

Immunofluorescent analysis of paraformaldehyde-fixed interphase HeLa cells labeling VRK1 with ab171933 at 1/100 dilution (center). DNA was stained with DAPI (left) and two images were merged (right; merge).

Immunofluorescent analysis of paraformaldehyde-fixed metaphase HeLa cells labeling VRK1 with ab171933 at 1/100 dilution (center). DNA was stained with DAPI (left) and two images were merged (right; merge).

Immunofluorescent analysis of methanol-fixed interphase HeLa cells labeling VRK1 with ab171933 at 1/100 dilution (center). DNA was stained with DAPI (left) and two images were merged (right; merge).

Immunofluorescent analysis of methanol-fixed metaphase HeLa cells labeling VRK1 with ab171933 at 1/100 dilution (center). DNA was stained with DAPI (left) and two images were merged (right; merge).Metaphase cells. At metaphase, VRK1 dots were solely detected in nuclei.

Immunofluorescent analysis of formaldehyde-fixed interphase U-2 OS cells labeling VRK1 with ab171933 at 1/100 dilution (center). DNA was stained with DAPI (left) and two images were merged (right; merge).

Immunofluorescent analysis of formaldehyde-fixed metaphase U-2 OS cells labeling VRK1 with ab171933 at 1/100 dilution (center). DNA was stained with DAPI (left) and two images were merged (right; merge).Metaphase cells. At metaphase, VRK1 dots were solely detected in nuclei.

Immunofluorescent analysis of methanol-fixed interphase U-2 OS cells labeling VRK1 with ab171933 at 1/100 dilution (center). DNA was stained with DAPI (left) and two images were merged (right; merge).

Immunofluorescent analysis of methanol-fixed metaphase U-2 OS cells labeling VRK1 with ab171933 at 1/100 dilution (center). DNA was stained with DAPI (left) and two images were merged (right; merge).Metaphase cells. At metaphase, VRK1 dots were solely detected in nuclei.