![Overlay histogram showing HEK293 cells stained with ab75591 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab75591, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/20199_VLDL-Receptor-Primary-antibodies-ab75591-2.jpg)
Overlay histogram showing HEK293 cells stained with ab75591 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab75591, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Flow cytometry analysis of Human THP-1 cell lines, staining VLDL Receptor (red histogram) with ab75591. Cells were taken from suspension culture and washed with PBS/BSA before fixation. Cells were blocked and permeabilized in 10% donkey serum/0.3 M glycine/0.1% BSA/0.1% Tween-20 in PBS for 20 mins at room temperature. The sample was incubated with the primary antibody (2 µg/106 cells in 0.1% BSA/0.1% Tween-20 in PBS) for 30 minutes at 22°C. A FITC-conjugated donkey anti-mouse polyclonal IgG (30 µg/ml) was used as the secondary antibody.Gating Strategy: FSC/SSCSee Abreview