Anti-Vitamin D Receptor antibody - ChIP Grade
| Name | Anti-Vitamin D Receptor antibody - ChIP Grade |
|---|---|
| Supplier | Abcam |
| Catalog | ab3508 |
| Prices | $401.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF IHC-F EMSA IP WB ChIPseq IHC-P ChIP |
| Species Reactivities | Mouse, Rat, Chicken, Human, Bovine, Pig, Zebrafish |
| Antigen | Synthetic peptide corresponding to Human Vitamin D Receptor aa 395-413 |
| Description | Rabbit Polyclonal |
| Gene | VDR |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Ab3508 staining Human normal jejunum. Staining is localized to the nucleus.Left panel: with primary antibody at 1/2000. Right panel: isotype control.Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer, citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
ab3508 staining the Vitamin D Receptor in mVDR-transfected and untransfected Mouse NIH/3T3 cells by ICC/IF (immunocytochemistry/immunofluorescence). Cells were paraformaldehyde fixed, permeabilized with Triton X-100 and blocked with 1% BSA/5%HS for 30 minutes at 20°C. The sample was incubated with the primary antibody (1/50 in 1% BSA/5% HS in 1xPBS) for 1 hour 30 minutes at 20°C. A Cy3®-conjugated goat anti-rabbit polyclonal (1/200) was used as the secondary.See Abreview
ab3508 at a 1/2000 dilution staining Vitamin D Receptor in whole Rat embryo tissue sections by Immunohistochemistry (frozen sections) incubated for 16 hours at 25°C. Samples fixed in 4% PFA prior to cutting at 30µm thickness. Blocked with 5% serum for 1 hour at 25°C. Secondary used at 1/200 polyclonal Goat anti-rabbit conjugated to Alexa Fluor 488.See Abreview
Product References
Vitamin D receptor regulates TGF-beta signalling in systemic sclerosis. - Vitamin D receptor regulates TGF-beta signalling in systemic sclerosis.
Zerr P, Vollath S, Palumbo-Zerr K, Tomcik M, Huang J, Distler A, Beyer C, Dees C, Gela K, Distler O, Schett G, Distler JH. Ann Rheum Dis. 2015 Mar;74(3):e20.
Role of local bioactivation of vitamin D by CYP27A1 and CYP2R1 in the control of - Role of local bioactivation of vitamin D by CYP27A1 and CYP2R1 in the control of
Bergada L, Pallares J, Maria Vittoria A, Cardus A, Santacana M, Valls J, Cao G, Fernandez E, Dolcet X, Dusso AS, Matias-Guiu X. Lab Invest. 2014 Jun;94(6):608-22.
Immunohistochemical expression of VDR is associated with reduced integrity of - Immunohistochemical expression of VDR is associated with reduced integrity of
Visconti B, Paolino G, Carotti S, Pendolino AL, Morini S, Richetta AG, Calvieri S. J Eur Acad Dermatol Venereol. 2014 Sep 15.
Molecular network of chromatin immunoprecipitation followed by deep - Molecular network of chromatin immunoprecipitation followed by deep
Satoh J, Tabunoki H. Mult Scler. 2013 Jul;19(8):1035-45.
Calcitriol imparts neuroprotection in vitro to midbrain dopaminergic neurons by - Calcitriol imparts neuroprotection in vitro to midbrain dopaminergic neurons by
Orme RP, Bhangal MS, Fricker RA. PLoS One. 2013 Apr 23;8(4):e62040.
Pharmacology of AMG 416 (Velcalcetide), a novel peptide agonist of the - Pharmacology of AMG 416 (Velcalcetide), a novel peptide agonist of the
Walter S, Baruch A, Dong J, Tomlinson JE, Alexander ST, Janes J, Hunter T, Yin Q, Maclean D, Bell G, Mendel DB, Johnson RM, Karim F. J Pharmacol Exp Ther. 2013 Aug;346(2):229-40.
Argonaute 2 sustains the gene expression program driving human monocytic - Argonaute 2 sustains the gene expression program driving human monocytic
Iosue I, Quaranta R, Masciarelli S, Fontemaggi G, Batassa EM, Bertolami C, Ottone T, Divona M, Salvatori B, Padula F, Fatica A, Lo-Coco F, Nervi C, Fazi F. Cell Death Dis. 2013 Nov 21;4:e926.
BRCA2 protein deficiency exaggerates doxorubicin-induced cardiomyocyte apoptosis - BRCA2 protein deficiency exaggerates doxorubicin-induced cardiomyocyte apoptosis
Singh KK, Shukla PC, Quan A, Desjardins JF, Lovren F, Pan Y, Garg V, Gosal S, Garg A, Szmitko PE, Schneider MD, Parker TG, Stanford WL, Leong-Poi H, Teoh H, Al-Omran M, Verma S. J Biol Chem. 2012 Feb 24;287(9):6604-14.
KDM6B/JMJD3 histone demethylase is induced by vitamin D and modulates its effects - KDM6B/JMJD3 histone demethylase is induced by vitamin D and modulates its effects
Pereira F, Barbachano A, Silva J, Bonilla F, Campbell MJ, Munoz A, Larriba MJ. Hum Mol Genet. 2011 Dec 1;20(23):4655-65.
Epigenetic control of a VDR-governed feed-forward loop that regulates - Epigenetic control of a VDR-governed feed-forward loop that regulates
Thorne JL, Maguire O, Doig CL, Battaglia S, Fehr L, Sucheston LE, Heinaniemi M, O'Neill LP, McCabe CJ, Turner BM, Carlberg C, Campbell MJ. Nucleic Acids Res. 2011 Mar;39(6):2045-56.