ab195878 staining Vimentin in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab195878 at 1/5000 dilution(shown in red) and ab6046, Rabbit polyclonal to beta Tubulin at 1µg/ml overnight at +4°C. ab150081, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed, was then incubated at 2µg/ml for 1h at room temperature (shown in green) Nuclear DNA was labelled in blue with DAPI.This product gave a positive signal in 100% methanol (5 min) fixed HeLa cells under the same testing conditions.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).