ICC/IF image of VE Cadherin stained HUVEC cells. The cells The cells were incubated with the antibody ab33168 at 1/150 (Green). The cells were also stained with Rhodamine phalloidin (Red).

ab33168 (1/50) staining VE Cadherin in paraffin-embbeded mouse heart tissue sections. Tissue underwent fixation in formaldehyde, heat-mediated antigen retrieval in citrate buffer and blocking (5 minutes/peroxidase block and 10 minutes/protein block). For further experimental details please refer to abreview.See Abreview

Anti-VE Cadherin antibody - Intercellular Junction Marker (ab33168) at 1 µg/ml + HUVEC Cell Lysate at 20 µgSecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilutionPerformed under reducing conditions.

ab33168 used in Flow cytometry.Human REH B cells were fixed in paraformaldehyde and permeabilized using saponin. Primary antibody used undiluted (2µl in 100µl of cells in PBS) and incubated for 15 minutes at 4°C. The secondary antibody used was an undiluted, Alexa Fluor®488 conjugated goat anti-rabbit IgG.Rabbit IgG isotype control (white)See Abreview

ab33168 Immunoprecipitating VE Cadherin in human HUVEC whole cell lysate. 1000000 cells lysate was incubated with primary antibody (1/100 in 0.5% NP40, 150mM NaCl, 50mM Tris) and matrix (Dynabeads) for 2 hours at 4°C. For western blotting a HRP-conjugated mouse anti-VE Cadherin (1/3000) was used to confirm successful immunoprecipation.See Abreview