Anti-VCP antibody

Name	Anti-VCP antibody
Supplier	Abcam
Catalog	ab36047
Prices	$376.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Applications	WB IHC-P
Species Reactivities	Mouse, Rat, Human, Bovine, Pig
Antigen	Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human VCP
Description	Rabbit Polyclonal
Gene	VCP
Conjugate	Unconjugated
Supplier Page	Shop

Product images

All lanes : Anti-VCP antibody (ab36047) at 1 µg/mlLane 1 : HL60 (Human promyelocytic leukemia cell line) Whole Cell LysateLane 2 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate Lysates/proteins at 10 µg per lane.SecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilutionPerformed under reducing conditions.

All lanes : Anti-VCP antibody (ab36047) at 1 µg/mlLane 1 : Kidney (Mouse) Tissue Lysate Lane 2 : Lung (Mouse) Whole Cell Lysate - normal tissue (ab29297)Lane 3 : Liver (Mouse) Tissue Lysate Lane 4 : Kidney (Rat) Tissue Lysate Lane 5 : Lung (Rat) Tissue Lysate Lane 6 : Liver (Rat) Tissue Lysate Lysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

IHC image of ab36047 staining in Human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab36047, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.