![All lanes : Anti-VAMP2 antibody [EPR12790] (ab181869) at 1/50000 dilutionLane 1 : Human fetal brain lysateLane 2 : Human cerebellum lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/19057_ab181869-216237-ab181869-WB.jpg)
All lanes : Anti-VAMP2 antibody [EPR12790] (ab181869) at 1/50000 dilutionLane 1 : Human fetal brain lysateLane 2 : Human cerebellum lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Immunofluorescent analysis of SH-SYSY cells (4% paraformaldehyde-fixed) labeling VAMP2 with ab181869 at 1/500 dilution followed by Goat anti rabbit IgG (Alexa Fluor®488) at 1/200 dilution and counterstained with Dapi.

Western blot analysis of immunoprecipitation pellet from Human cerebellum lysate immunoprecipitated using ab181869 at 1/70 dilution.Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.

Flow cytometry analysis of Jurkat cells fixed with 2% paraformaldehyde labeling VAMP2 with ab181869 at 1/150 dilution followed by Goat anti rabbit IgG (FITC) at 1/150 dilution. Rabbit monoclonal IgG was used as an isotype control.

Immunofluorescent analysis of U87-MG cells (4% paraformaldehyde-fixed) labeling VAMP2 with ab181869 at 1/500 dilution followed by Goat anti rabbit IgG (Alexa Fluor®488) at 1/200 dilution and counterstained with Dapi.