ab837 at a dilution of 1/200, staining Ubiquitin in neurons (Alexa 488 secondary at 1/2000) on 30µm coronal rat brain tissue sections in free floating IHC (see protocol link for detailed description). Image shows neuronal staining observed with 40x objective. The background is quite high at this concentration of ab837 and we would recommend a working dilution of 1/500-1/750NB: No labeling observed following omission of primary antibody.Sections were viewed using an Axioplan 2 Imaging microscope (Imaging Associates) fitted with 10x, 20x and 40x Plan-Neofluorobjectives (Zeiss, Germany) and images were taken using a AxioCam Hrm digital camera (Zeiss, Germany) and AxioVision software (Imaging Associates).