Central Panel: ab14372 staining Ubiquitin in human PMN cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 2% BSA for 1 hour. Samples were incubated with primary antibody 1/100 in blocking buffer for 4 hours at 37°C. An Alexa Fluor® 568-conjugated Goat polyclonal to rabbit IgG, dilution 1/250, was used as secondary antibody. Left-hand panel: Nuclei counterstained with DAPI (blue).Right-hand panel: OverlaySee Abreview

ab14372 staining Ubiquitin in Human platelet cells by Flow cytometry.Cells were fixed in paraformaldehyde and permeabilized using 0.1% Triton-X-100 in 2% BSA for 15 minutes. Primary antibody used at a 1/200 dilution and incubated for 18 hours at 4°C. The secondary antibody used was an Alexa Fluor®488 conjugated chicken anti-rabbit IgG (H+L) at a 1/500 dilution. P : Permeabilized; US : Unstained, Red Peak; IGG RB : IgG Rabbit (Isotype Control), Blue Peak; Ubiquitin, Green peak.See Abreview