Anti-Ubiquitin antibody
| Name | Anti-Ubiquitin antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab7780 |
| Prices | $400.00 |
| Sizes | 500 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF IHC-F IHC-P WB IP |
| Species Reactivities | Mouse, Rat, Human, Monkey, Horse, Bovine |
| Antigen | Recombinant full length protein (Human) |
| Description | Rabbit Polyclonal |
| Gene | UBB |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
developed using the ECL technique
All lanes : Anti-Ubiquitin antibody (ab7780) at 1/2000 dilutionLane 1 : Human Panc-1 whole cell lysateLane 2 : Human Panc-1 whole cell lysateLysates/proteins at 700 µg per lane.SecondaryHRP Donkey anti-rabbit IgG polyclonal at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
ab7780 staining Ubiquitin in Panc-1 cells by Immunocytochemistry/ Immunofluorescence.Cells were fixed in formaldehyde, blocked with 1% serum for 1 hour at 22°C and then incubated with ab7780 at a 1/2000 dilution for 1 hour at 22°C. The secondary was used at a 1/200 dilution.Green - Golgi residental proteins C2GnT-M.Red -Ubiquitin.Blue - nucleus staining by DAPI.See Abreview
ICC/IF image of ab7780 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum (ab7481) / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7780, 1æg/ml) overnight at +4øC. The secondary antibody (green)ÿwas Alexa Fluor© 488 goat anti-mouse IgG (H+L) ab150113) used at a 1/1000 dilution for 1h. Alexa Fluor© 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43æM.
![ab7780 at a dilution of 1/1000, staining Ubiquitin in neurons (Alexa 488 secondary at 1/2000) on 30µm coronal rat brain (ab29475) tissue sections in free floating IHC (see protocol link for detailed description). Image shows neuronal staining observed with [A] 20x objective and [B] 40x objective.NB: No labeling observed following omission of primary antibody.Sections were viewed using an Axioplan 2 Imaging microscope (Imaging Associates) fitted with 10x, 20x and 40x Plan-Neofluorobjectives (Zeiss, Germany) and images were taken using a AxioCam Hrm digital camera (Zeiss, Germany) and AxioVision software (Imaging Associates).](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/17489_ab7780_1.jpg)
ab7780 at a dilution of 1/1000, staining Ubiquitin in neurons (Alexa 488 secondary at 1/2000) on 30µm coronal rat brain (ab29475) tissue sections in free floating IHC (see protocol link for detailed description). Image shows neuronal staining observed with [A] 20x objective and [B] 40x objective.NB: No labeling observed following omission of primary antibody.Sections were viewed using an Axioplan 2 Imaging microscope (Imaging Associates) fitted with 10x, 20x and 40x Plan-Neofluorobjectives (Zeiss, Germany) and images were taken using a AxioCam Hrm digital camera (Zeiss, Germany) and AxioVision software (Imaging Associates).
ab7780 (1/100) staining Ubiquitin in paraffin-embedded brain sections of a patient with Alzheimer's disease.
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