Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034]

Name	Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034]
Supplier	Abcam
Catalog	ab181537
Prices	$99.00, $325.00
Sizes	10 µl, 100 µl
Host	Rabbit
Clonality	Monoclonal
Isotype	IgG
Clone	EPR17034
Applications	IHC-P WB FC
Species Reactivities	Mouse, Rat, Human
Antigen	Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Ubiquitin aa 1-100 (linkage-specific K27)
Description	Rabbit Monoclonal
Gene	UBB
Conjugate	Unconjugated
Supplier Page	Shop

Product images

All lanes : Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (ab181537) at 1/5000 dilutionLane 1 : K27-linked-Ub2 recombinant proteinLane 2 : K6-linked-Ub2 recombinant proteinLane 3 : K11-linked-Ub2 recombinant proteinLane 4 : K29-linked-Ub2 recombinant proteinLane 5 : K33-linked-Ub2 recombinant proteinLane 6 : K48-linked-Ub2 recombinant proteinLane 7 : K63-linked-Ub2 recombinant proteinLane 8 : Ubiquitin recombinant proteinLane 9 : Ubiquitin linear recombinant proteinLysates/proteins at 0.02 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

All lanes : Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (ab181537) at 1/50000 dilutionLane 1 : 293T (Human epithelial cells from embryonic kidney) whole cell lysatesLane 2 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysatesLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (ab181537) at 1/5000 dilution + HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysates at 10 µgSecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

All lanes : Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (ab181537) at 1/5000 dilutionLane 1 : Mouse brain lysatesLane 2 : Mouse heart lysatesLane 3 : Mouse kidney lysatesLane 4 : Mouse spleen lysatesLane 5 : Rat brain lysatesLane 6 : Rat heart lysatesLane 7 : Rat kidney lysatesLane 8 : Rat spleen lysatesLane 9 : C6 (Rat glial tumor cells) whole cell lysatesLane 10 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysatesLane 11 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysatesLane 12 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysatesLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Immunohistochemical analysis of paraffin-embedded Human transitional cell carcinoma of bladder labeling Ubiquitin (linkage-specific K27) with ab181537 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining on cancer cells of Human bladder transitional cell carcinoma is observed. Counter stained with Hematoxylin.Negative control: Used PBS instead of primary antibody.

Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling Ubiquitin (linkage-specific K27) with ab181537 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining on lymphocytes of Mouse spleen is observed. Counter stained with Hematoxylin.Negative control: Used PBS instead of primary antibody.

Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling Ubiquitin (linkage-specific K27) with ab181537 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining on lymphocytes of Rat spleen is observed. Counter stained with Hematoxylin.Negative control: Used PBS instead of primary antibody.

Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling Ubiquitin (linkage-specific K27) with ab181537 at 1/160 dilution (red). The secondary antibody was Goat anti rabbit IgG (FITC) at 1/150 dilution. The isotype control is Rabbit monoclonal IgG (black) and cell without incubation with primary antibody and secondary antibody is blue.