![All lanes : Anti-Twist antibody [10E4E6] (ab175430) at 1/500 dilutionLane 1 : HEK293 cell lysateLane 2 : Twist (aa9-74) - hIgGFc transfected HEK293 cell lysate](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/16490_ab175430-203008-8.jpg)
All lanes : Anti-Twist antibody [10E4E6] (ab175430) at 1/500 dilutionLane 1 : HEK293 cell lysateLane 2 : Twist (aa9-74) - hIgGFc transfected HEK293 cell lysate
![Anti-Twist antibody [10E4E6] (ab175430) at 1/500 dilution + Twist recombinant protein](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/16491_ab175430-203007-7.jpg)
Anti-Twist antibody [10E4E6] (ab175430) at 1/500 dilution + Twist recombinant protein
![All lanes : Anti-Twist antibody [10E4E6] (ab175430) at 1/500 dilutionLane 1 : NIH/3T3 cell lysateLane 2 : Jurkat cell lysateLane 3 : HeLa cell lysateLane 4 : A549 cell lysateLane 5 : Raji cell lysateLane 6 : OCM-1 cell lysate](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/16492_ab175430-203006-6.jpg)
All lanes : Anti-Twist antibody [10E4E6] (ab175430) at 1/500 dilutionLane 1 : NIH/3T3 cell lysateLane 2 : Jurkat cell lysateLane 3 : HeLa cell lysateLane 4 : A549 cell lysateLane 5 : Raji cell lysateLane 6 : OCM-1 cell lysate

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue labeling Twist with ab175430 at 1/200 dilution, with DAB staining.

Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue labeling Twist with ab175430 at 1/200 dilution, with DAB staining.

Immunofluorescent analysis of Hela cells labeling Twist with ab175430 at 1/200 dilution (green). Blue: DRAQ5 fluorescent DNA dye.

Flow cytometric analysis of HeLa cells labeling Twist with ab175430 at 1/200 dilution (green) compared to a negative control (red).