All lanes : Anti-TUBA4A (acetyl K40) antibody [6-11B-1] (ab11323) at 1/1000 dilutionLane 1 : Whole animal lysate prepared from wild type (N2) C. Elegans worms.Lane 2 : Whole animal lysate prepared from elpc-1 C. Elegans worms.Lane 3 : Whole animal lysate prepared from elpc-3 C. Elegans worms.Lane 4 : Whole animal lysate prepared from mec-12 (e1605) C. Elegans worms.Lane 5 : Whole animal lysate prepared from mec-12 (e1607) C. Elegans worms.Lysates/proteins at 20 µg per lane.Image from Chen C et al, PLoS Genet. 2009 Jul;5(7):e1000561. Epub 2009 Jul 10, fig S9.

Anti-TUBA4A (acetyl K40) antibody [6-11B-1] (ab11323) at 1/2000 dilution (in PBST + 2.5% milk for 16 hours at 4°C) + Whole cell lysate of human IHP1 cells at 30 µgSecondaryAn HRP-conjugated Goat anti-mouse IgG monoclonal at 1/2000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Observed band size : 55 kDa (why is the actual band size different from the predicted?)Exposure time : 20 secondsThis image is courtesy of an anonymous AbreviewSee Abreview

ab11323 staining Tubulin (acetyl K40) in human glioblastoma tumor sphere cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 0.1% in PBS and blocked with 0.5% BSA for 20 minutes at room temperature. Samples were incubated with primary antibody (1/100 in PBS + 0.5% BSA) for 1 hour at 4°C. An Alexa Fluor®488-conjugated Goat anti-mouse IgG polyclonal (1/400) was used as the secondary antibody. Nuclei were counterstained with DAPI (blue).See Abreview