All lanes : Anti-TTC19 antibody [EPR13184-63] (ab181191) at 1/5000 dilutionLane 1 : Human ovarian cancer lysateLane 2 : HT-1080 lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution (Goat Anti-Rabbit IgG HRP)
Immunocytochemical analysis of HT-1080 cells fixed in 4% paraformaldehyde labeling TTC19 with ab181191 at 1/100 dilution and Goat anti rabbit IgG (Alexa Fluor® 488) at 1/200 dilution (green). Counterstained with DAP1 (blue)
Immunoprecipitation of HT-1080 cells labeling TTC19 with ab181191 at 1/50 dilution and Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution.