![Anti-Troponin I fast skeletal muscle antibody [EPR14927(B)] (ab184554) at 1/20000 dilution + Human skeletal muscle lysate at 20 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/15162_ab184554-216803-184554.JPG)
Anti-Troponin I fast skeletal muscle antibody [EPR14927(B)] (ab184554) at 1/20000 dilution + Human skeletal muscle lysate at 20 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
![All lanes : Anti-Troponin I fast skeletal muscle antibody [EPR14927(B)] (ab184554) at 1/20000 dilutionLane 1 : Mouse muscle lysateLane 2 : Rat muscle lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/15163_ab184554-216802-1845542.JPG)
All lanes : Anti-Troponin I fast skeletal muscle antibody [EPR14927(B)] (ab184554) at 1/20000 dilutionLane 1 : Mouse muscle lysateLane 2 : Rat muscle lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Western blot analysis of Troponin I fast skeletal muscle in Human skeletal muscle lysate immunoprecipitated using ab184554 at 1/50 dilution (Lane 1). Lane 2: PBS instead of Human skeletal muscle lysate.Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.