Anti-Topoisomerase II beta antibody [EPR5377] (Alexa Fluor® 647)
| Name | Anti-Topoisomerase II beta antibody [EPR5377] (Alexa Fluor® 647) |
|---|---|
| Supplier | Abcam |
| Catalog | ab200993 |
| Prices | $425.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | EPR5377 |
| Applications | ICC/IF ICC/IF |
| Species Reactivities | Human, Mouse, Rat |
| Antigen | Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Topoisomerase II beta aa 50-150 |
| Description | Rabbit Monoclonal |
| Gene | TOP2B |
| Conjugate | Alexa Fluor® 647 |
| Supplier Page | Shop |
Product images
ab200933 staining Topoisomerase II beta in HeLa cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab200933 at 1/200 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 2µg/ml (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).