All lanes : Anti-TNFAIP8 antibody [EPR10058(3)] (ab195810) at 1/1000 dilutionLane 1 : K562 cell lysateLane 2 : A549 cell lysateLane 3 : A431 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Anti-TNFAIP8 antibody [EPR10058(3)] (ab195810) at 1/1000 dilution + Human fetal spleen lysate at 20 µgSecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
TNFAIP8 was immunoprecipitated from K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell extract with ab195810 at 1/70 dilution (Lane 2). Western blot was performed from the immunoprecipitate using ab195810 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution. Lane 1: K562 whole cell extract (Input) 10µg. Lane 2: ab195810 IP in K562 whole cell extract. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab195810 in K562 whole cell extract.Blocking and dilution buffer and concentration: 5% NFDM/TBST.