All lanes : Anti-TLR7 antibody [EPR2088(2)] (ab124928) at 1300 (purified)Lane 1 : Ramos cell lysateLane 2 : Daudi cell lysateLysates/proteins at 20 µg per lane.SecondaryPeroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

All lanes : Anti-TLR7 antibody [EPR2088(2)] (ab124928) at 1/1000 dilution (unpurified)Lane 1 : Raji cell lysatesLane 2 : Ramos cell lysatesLysates/proteins at 10 µg per lane.SecondaryHRP-conjugated goat anti-rabbit at 1/2000 dilution

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling TLR7 with unpurified ab124928 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with hematoxylin.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling TLR7 with purified ab124928 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with hematoxylin.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling TLR7 with unpurified ab124928 at 1/100.

Equilibrium disassociation constant (KD)Learn more about KD Click here to learn more about KD