Anti-TIE2 antibody [Cl. 16]
| Name | Anti-TIE2 antibody [Cl. 16] |
|---|---|
| Supplier | Abcam |
| Catalog | ab24859 |
| Prices | $400.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | Cl. 16 |
| Applications | IHC-F IHC-F WB ELISA FC IHC-P |
| Species Reactivities | Mouse, Human |
| Antigen | Recombinant fragment corresponding to Human TIE2 |
| Description | Mouse Monoclonal |
| Gene | TEK |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Overlay histogram showing JEG-3 cells stained with ab24859 (red line). The cells were fixed with methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab24859, 2µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a decreased signal in JEG-3 cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.Please note that Abcam does not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/11075_TIE2-Primary-antibodies-ab24859-2.jpg)
Overlay histogram showing JEG-3 cells stained with ab24859 (red line). The cells were fixed with methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab24859, 2µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a decreased signal in JEG-3 cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.Please note that Abcam does not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.
Immunohistochemical analysis of Human brain tissue, staining TIE2 with ab24859.Tissue was fixed with paraformaldehyde, permeabilized with 0.25 Triton X-100 and blocked with 2.5% BSA for 30 minutes at 25°C. Samples were incubated with primary antibody (1/200 in 2.5% horse serum) for 18 hours at 4°C. An HRP-conjugated horse anti-rabbit polyclonal IgG was used as the secondary antibody. See Abreview
ab24859 staining TIE2 in Human spleen by Immunohistochemistry (Frozen sections).
![All lanes : Anti-TIE2 antibody [Cl. 16] (ab24859)Lane 1 : HUVECs left untreatedLane 2 : HUVECs stimulated for 3 hours with PMA at 25 ng/mlLane 3 : HUVECs stimulated for 6 hours with PMA at 25 ng/mlLane 4 : HUVECs stimulated for 9 hours with PMA at 25 ng/mlLane 5 : HUVECs stimulated for 24 hours with PMA at 25 ng/ml](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/11078_TIE2-Primary-antibodies-ab24859-4.jpg)
All lanes : Anti-TIE2 antibody [Cl. 16] (ab24859)Lane 1 : HUVECs left untreatedLane 2 : HUVECs stimulated for 3 hours with PMA at 25 ng/mlLane 3 : HUVECs stimulated for 6 hours with PMA at 25 ng/mlLane 4 : HUVECs stimulated for 9 hours with PMA at 25 ng/mlLane 5 : HUVECs stimulated for 24 hours with PMA at 25 ng/ml
developed using the ECL techniquePerformed under reducing conditions.
Product References
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