ab47183 staining TIE2 in Human HUVEC cells by Flow Cytometry. The sample was incubated with the primary antibody (1/10 in PBS + 1% BSA) for 1 hour at 4°C. A phycoerythrin-conjugated Goat anti-mouset IgG (1/100) was used as the secondary antibody.Gating Strategy: Live Cells using FSC and SSC.See Abreview