Immunohistochemical staining of paraffin embedded human thyroid with purified ab133322 at a working dilution of 1 in 1000. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

ab133322 (purified) at 1/40 immunoprecipitating thyroid peroxidase in human thyroid tissue lysate (Lane 1). For western blotting, a HRP-conjugated goat anti-rabbit IgG, was used as the secondary antibody (1/1000).Blocking buffer and concentration: 5% NFDM/TBST.Diluting buffer and concentration: 5% NFDM /TBST.

Anti-Thyroid Peroxidase antibody [EPR5379] (ab133322) at 1/1000 dilution (unpurified) + Human thyroid lysate at 10 µgSecondaryHRP labelled goat anti-rabbit at 1/2000 dilution