Anti-TFIIB (acetyl K238) antibody
| Name | Anti-TFIIB (acetyl K238) antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab5210 |
| Prices | $242.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human TFIIB, acetylated at K238 |
| Blocking Peptide | Human TFIIB (acetyl K238) peptide |
| Description | Rabbit Polyclonal |
| Gene | GTF2B |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
This antibody gave a positive result in ELISA against the immunising peptide (ab26407) and a negative result using the non-modified equivalent peptide (ab25428). This indicates that it is specific for the modified peptide. Red line: ELISA against immunising peptide ab26407 Green line: ELISA against non-modified peptide ab25428
ICC/IF image of ab5210 stained HeLa cells. The cells were 100% methanol fixed (5 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab5210, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in 4% PFA and 100% methanol fixed Hek293, HepG2 and Mcf7 cells. However, this Fast-Track antibody is not yet fully characterised. This image represents inconclusive preliminary data.