Immunofluorescence staining of TAF1 in HeLa cells with ab188427 at a 1/5000 dilution. Hela cells were fixed with 4% paraformaldehyde and permeabilized with 0.25% Triton X-100.
Anti-TAF1 antibody (ab188427) at 1/1000 dilution + HeLa whole cell extract at 10 µg
IImmunofluorescence staining of TAF1 in in BHK cells (fixed with 3% formaldehyde and permiabilized 1% Nonidet P-40) with ab188427 at 1/1000 dilution. Secondary staining with Goat anti-Rabbit IgG conjugated with rhodamine.Panel a): Stained with ab188427 without immunizing peptide.Panel b): Stained with ab188427 with immunizing peptide.Panel c): Panel a) co-stained with Hoechst 33258. Panel d): Panel b) co-stained with Hoechst 33258.Panel e): Phase-contrast image of panels a) and c).Panel f): Phase-contrast image of panels b) and d).