Anti-TACC3 antibody [EPR7756] (ab134154) at 1/10000 dilution (purified) + Human lymph node tissue lysate at 10 µgSecondaryPeroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

All lanes : Anti-TACC3 antibody [EPR7756] (ab134154) at 1/10000 dilution (purified)Lane 1 : Mouse testis tissue lysateLane 2 : Rat testis tissue lysateLysates/proteins at 20 µg per lane.SecondaryPeroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

All lanes : Anti-TACC3 antibody [EPR7756] (ab134154) at 1/1000 dilution (unpurified)Lane 1 : Human lymph node tissue lysateLane 2 : HT-29 cell lysateLane 3 : HeLa cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling TACC3 with purified ab134154 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with Hematoxylin.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling TACC3 with unpurified ab134154 at 1/50 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling TACC3 with unpurified ab134154 at 1/50 dilution.

Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling TACC3 with purified ab134154 at 1/200. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. An Alexa Fluor® 555-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.Control: primary antibody (1/200) and secondary antibody, ab150113, an Alexa Fluor® 488-conjugated goat anti-mouse IgG (1/500).

Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling TACC3 with unpurified ab134154 at 1/50 dilution.