Immunocytochemistry with SH-SY5Y cells stained with Anti-SYT2 antibody (ab154035) in red and DAPI in blue, as a counter stain. The target protein locates to the plasma membrane (red). Fixation: 4% Paraformaldehyde, 20 min. Permeabilization: 0.1% Triton X-100, 10min. Blocking: 10% Goat Serum/PBS, 1 hour. Primary: Anti-SYT2 antibody (ab154035) 5 µg/ml in 10% Goat Serum/PBS, 2hr at room temperature or over night at 4°C. Secondary: Alexa Fluor® 594 Goat Anti-Mouse (H&L) used at a 1/1000 dilution in 10% Goat Serum/PBS, 1hr at RT. Washing: 3x 1% Goat Serum/PBS, 10 mins/wash DAPI: 2 µM in 1% Goat Serum/PBS, 10 mins