All lanes : Anti-Synaptopodin 2 antibody (ab103710) at 1 µg/mlLane 1 : Skeletal Muscle (Mouse) Tissue LysateLane 2 : Skeletal Muscle (Rat) Tissue LysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Observed band size : 100 kDa (why is the actual band size different from the predicted?)Additional bands at : 18 kDa,55 kDa. We are unsure as to the identity of these extra bands.Exposure time : 3 minutes