Stromal interaction molecule 1 antibody (ab57834) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human malignant lymphoma, diffuse large B.
ab57834 at 10 ug/ml staining Stromal interaction molecule 1 in human Hella cells by Immunocytochemistry/ Immunofluorescence.
ab57834 staining the stromal interaction molecule in HeLa cells by immunocytochemistry/immunofluorescence (ICC/IF). Cells were fixed with paraformaldehyde and permeabilized with 0.5% TritonX. Samples were incubated with primary antibody (1/200) for 1 hour at 24°C. An Alexa Fluor ® 488-conjugated chicken anti-mouse polyclonal (1/1000) was used as the secondary.See Abreview
![Overlay histogram showing HeLa cells stained with ab57834 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57834, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/6504_Stromal-interaction-molecule-1-Primary-antibodies-ab57834-32.jpg)
Overlay histogram showing HeLa cells stained with ab57834 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57834, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
