All lanes : Anti-Striatin 4 antibody [EPR11801] (ab177155) at 1/1000 dilutionLane 1 : Human fetal brain lysateLane 2 : K562 lysateLane 3 : U87-MG lysateLane 4 : Human cerebellum lysateLane 5 : Human fetal lung lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP at 1/2000 dilution
Immunofluorescent analysis of U87-MG cells labeling Striatin 4 with ab177155 at 1/50 dilution.
Western blot analysis on immunoprecipitation pellet from Human fetal brain lysate, immunoprecipitated using ab177155 at 1/10 dilution.
Flow cytometric analysis of permeabilized K562 cells labeling Striatin 4 with ab177155 at 1/10 dilution (red), or a rabbit IgG (negative) (green).