All lanes : Anti-STARD5 antibody [EPR13545(B)] (ab178688) at 1/1000 dilutionLane 1 : HepG2 lysatesLane 2 : 293T lysatesLane 3 : THP-1 lysatesLane 4 : Human fetal liver lysatesLane 5 : Human fetal kidney lysatesLane 6 : HL-60 lysatesLysates/proteins at 10 µg per lane.
Flow cytometric analysis of permeabilized 293T cells using ab178688 at a 1/10 dilution (red) or a rabbit IgG (negative) (green).
Western blot analysis on immunoprecipitation pellet from (Lane 1) THP-1 cell lysate or (Lane 2) 1X PBS (negative control) using ab178688 at a 1/10 dilution for IP and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.