Immunofluorescent staining of Human cell line U-251MG shows positivity in nuclei but not nucleoli. Recommended concentration of ab121594 1-4 µg/ml. Cells treated with PFA/Triton X-100.
ab121594, at a 1/50 dilution, staining SPATA4 in paraffin embedded Human testis tissue by Immunohistochemistry.
All lanes : Anti-SPATA4 antibody (ab121594) at 1/250 dilutionLane 1 : Molecular weight markerLane 2 : RT-4 lysateLane 3 : U251-MG lysateLane 4 : Human plasma lysateLane 5 : Human liver lysateLane 6 : Human tonsil lysate