![All lanes : Anti-SPAG7 antibody [EPR13391-33] (ab181184) at 1/2000 dilutionLane 1 : MCF7 cell lysateLane 2 : SH-SY5Y cell lysateLane 3 : HeLa cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/4062_ab181184-214626-ab181184.jpg)
All lanes : Anti-SPAG7 antibody [EPR13391-33] (ab181184) at 1/2000 dilutionLane 1 : MCF7 cell lysateLane 2 : SH-SY5Y cell lysateLane 3 : HeLa cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Immunoprecipitation. ab181184 at 1/1000 labeling SPAG7 immunoprecipitated from HeLa cell lysate using ab181184 at 1/60.Lane 2: Control IgG.

Immunofluorescent analysis of MCF7 cells labeling SPAG7 with ab181184 at 1/250 with Goat anti rabbit IgG (Alexa Fluor®488) at 1/200 and DAPI staining (blue).