Anti-SP1 antibody (ab77441) at 5 µg/ml + IMR-32 cell lysate at 25 µgSecondaryGoat anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

Anti-SP1 antibody (ab77441) at 5 µg/ml + HeLa nuclear cell lysate at 25 µgSecondaryGoat anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

Anti-SP1 antibody (ab77441) at 5 µg/ml + NIH3T3 cell lysate at 25 µgSecondaryGoat anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

ab77441 at 10µg/ml staining SP1 in HeLa cells by Immunofluorescence.

ab77441 staining SP1 in human endometrium by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue.

Overlay histogram showing HeLa cells stained with ab77441 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab77441, 2µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a diminished signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween 20 used under the same conditions.