Anti-Sonic Hedgehog antibody [5H4]

Name	Anti-Sonic Hedgehog antibody [5H4]
Supplier	Abcam
Catalog	ab135240
Prices	$384.00
Sizes	100 µl
Host	Mouse
Clonality	Monoclonal
Isotype	IgG1
Clone	5H4
Applications	WB IHC-P FC
Species Reactivities	Mouse, Human, Monkey
Antigen	Purified Recombinant fragment, corresponding to amino acids 26-161 of Human Sonic Hedgehog expressed in E
Description	Mouse Monoclonal
Gene	SHH
Conjugate	Unconjugated
Supplier Page	Shop

Product images

Anti-Sonic Hedgehog antibody [5H4] (ab135240) at 1/500 dilution + Human Sonic Hedgehog recombinant protein

All lanes : Anti-Sonic Hedgehog antibody [5H4] (ab135240) at 1/500 dilutionLane 1 : HEK293 cell lysateLane 2 : HEK293 cell lysate, transfected with Sonic Hedgehog (amino acids 26-161)-hIgGFc

All lanes : Anti-Sonic Hedgehog antibody [5H4] (ab135240) at 1/500 dilutionLane 1 : LNCaP cell lysateLane 2 : HepG2 cell lysateLane 3 : PANC1 cell lysateLane 4 : HeLa cell lysateLane 5 : SK-N-SH cell lysateLane 6 : F9 cell lysateLane 7 : NIH 3T3 cell lysateLane 8 : COS7 cell lysate

Flow cytometric analysis of HeLa cells labelling Sonic Hedgehog with ab135240 at 1/200 dilution (green). Negative control (red).

Immunohistochemical analysis of Paraffin-embedded Human liver cancer tissue labelling Sonic Hedgehog with ab135240 at 1/200 dilution followed by DAB staining.