All lanes : Anti-Solo antibody [EPR13772-74] (ab192873) at 1/1000 dilutionLane 1 : Mouse brain tissue lysateLane 2 : Mouse kidney tissue lysateLane 3 : Rat brain tissue lysateLane 4 : Raw264.7 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
All lanes : Anti-Solo antibody [EPR13772-74] (ab192873) at 1/1000 dilutionLane 1 : Human fetal brain tissue lysateLane 2 : HepG2 cell lysateLysates/proteins at 20 µg per lane.SecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Immunofluorescence analysis of Neuro2a cells labeling Solo using ab192873 at 1/50 dilution (20 μg/ml). A Goat anti rabbit IgG (Alexa Fluor®488) at 1/400 dilution (ab150077) was used as secondary antibody. Cells were fixed with 4% paraformaldehyde and permealized with 0.1% triton X-100. Counterstain: DAPI.