All lanes : Anti-SNRPC [EPR16034] antibody (ab192028) at 1/5000 dilutionLane 1 : Jurkat cell lysateLane 2 : K562 cell lysateLane 3 : HeLa cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
All lanes : Anti-SNRPC [EPR16034] antibody (ab192028) at 1/10000 dilutionLane 1 : C6 cell lysateLane 2 : Raw 264.7 cell lysateLane 3 : PC-12 cell lysateLane 4 : NIH/3T3 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling SNRPC with ab192028 at 1/1000 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin. The negative control utilised PBS instead of primary antibody.
Immunohistochemical analysis of paraffin-embedded Human squamous cell carcinoma of cervix tissue labeling SNRPC with ab192028 at 1/1000 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin. The negative control utilised PBS instead of primary antibody.
Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling SNRPC with ab192028 at 1/1000 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin. The negative control utilised PBS instead of primary antibody.
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilised HeLa cells labeling SNRPC with ab192028 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/200 dilution (green). The nuclear counter stain is Dapi (blue). The two negative controls are ab192028 at 1/500 dilution followed by Goat anti mouse IgG (Alexa Fluor®594) at 1/500 dilution.
Western blot analysis of SNRPC in K562 lysate immunoprecipitated using ab192028 at 1/40 dilution (Lane 1). Lane 2: PBS instead of K562 lysate. Secondary antibody: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.Blocking/dilution buffer: 5% NFDM/TBST.