Western blot of rat brain (RB) lysate showing detection of ~94 kDa GRP94 using Anti-GRP94 (SPC-101). Lane 1: MW ladder. Lane 2: Anti-GRP94 (1:250). Load: 20 µg RB lysate per lane. Block: 5%25 milk + TBST 1hr at RT. Primary antibody: Rabbit Anti-GRP94 polyclonal antibody (SPC-101) incubated for 60 min at RT. Secondary antibody: Goat Anti-Mouse HRP antibody at 1:50-1:100 for 60 min at RT. Colour Development: TMB solution for 5 min at RT. Predicted/Observed size: ~ 94-100 kDa. Other band(s): ~50, ~75 kDa. SPC-101D