Anti-SLC25A12 antibody [EPR16294]

Name	Anti-SLC25A12 antibody [EPR16294]
Supplier	Abcam
Catalog	ab200201
Prices	$347.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Isotype	IgG
Clone	EPR16294
Applications	IP WB
Species Reactivities	Mouse, Rat, Human
Antigen	Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human SLC25A12 aa 600 to the C-terminus
Description	Rabbit Monoclonal
Gene	SLC25A12
Conjugate	Unconjugated
Supplier Page	Shop

Product images

All lanes : Anti-SLC25A12 antibody [EPR16294] (ab200201) at 1/5000 dilutionLane 1 : Human fetal heart lysateLane 2 : Human skeletal muscle lysateLysates/proteins at 20 µg per lane.SecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

All lanes : Anti-SLC25A12 antibody [EPR16294] (ab200201) at 1/1000 dilutionLane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysateLane 2 : A431 (Human epidermoid carcinoma) whole cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

All lanes : Anti-SLC25A12 antibody [EPR16294] (ab200201) at 1/1000 dilutionLane 1 : Mouse brain lysateLane 2 : Mouse heart lysateLane 3 : Mouse kidney lysateLane 4 : Rat brain lysateLane 5 : Rat heart lysateLane 6 : Rat kidney lysateLane 7 : C6 (Rat glial tumor cells) whole cell lysateLane 8 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysateLane 9 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysateLane 10 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

SLC25A12 was immunoprecipitated from 1mg of Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate with ab200201 at 1/40 dilution.Western blot was performed from the immunoprecipitate using ab200201 at 1/1000 dilution.Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.Lane 1: Jurkat whole cell lysate 10 µg (Input).Lane 2: ab200201 IP in Jurkat whole cell lysate.Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab200201 in Jurkat whole cell lysate.Blocking and dilution buffer and concentration: 5% NFDM/TBST.Exposure time: 30 second.

SLC25A12 was immunoprecipitated from 1mg of Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate with ab200201 at 1/40 dilution.Western blot was performed from the immunoprecipitate using ab200201 at 1/1000 dilution (Panel A) or ab192244 at 1/1000 dilution (Panel B).Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.Lane 1: Jurkat whole cell lysate 10 µg (Input).Lane 2: ab200201 IP in Jurkat whole cell lysate.Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab200201 in Jurkat whole cell lysate.Blocking and dilution buffer and concentration: 5% NFDM/TBST.Exposure time (Panel A and Panel B): 30 second.