
Anti-SHMT1 antibody (ab186130) at 1 µg/ml + Jurkat whole cell lysate at 50 µgdeveloped using the ECL technique

Anti-SHMT1 antibody (ab186130) at 1 µg/ml + NIH 3T3 whole cell lysate at 50 µgdeveloped using the ECL technique

Detection of SHMT1 in immunoprecipitates of Jurkat whole cell lysate (1 mg for IP, 20% of IP loaded) using ab186130 at 6 µg per reaction for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.Detection: Chemiluminescence with an exposure time of 30 seconds.