Western blot analysis of SEC61A in Daudi cell lysate immunoprecipitated using ab183046 at 1/50 dilution (Lane 1). Lane 2: Negative control.Secondary antibody: Anti-Rabbit IgG (HRP) specific to the non-reduced form of IgG at 1/1500 dilution.
![All lanes : Anti-SEC61A antibody [EPR14379] (ab183046) at 1/5000 dilutionLane 1 : Human fetal brain lysateLane 2 : Daudi cell lysateLane 3 : A431 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/27898_ab183046-216114-ab183046WBa.jpg)
All lanes : Anti-SEC61A antibody [EPR14379] (ab183046) at 1/5000 dilutionLane 1 : Human fetal brain lysateLane 2 : Daudi cell lysateLane 3 : A431 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Flow cytometric analysis of 2% paraformaldehyde-fixed Daudi cells labeling SEC61A with ab183046 at 1/100 dilution (pink) compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 diution.
Immunofluorescent analysis of 4% paraformaldehyde-fixed A431 cells labeling SEC61A with ab183046 at 1/250 dilution, followed by Goat anti rabbit IgG (Alexa Fluor®488) secondary antibody at 1/200 dilution. Counter stained with Dapi (blue).
Immunohistochemical analysis of paraffin-embedded Human infiltrating duct carcinoma of breast tissue labeling SEC61A with ab183046 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.