All lanes : Anti-S adenosylhomocysteine hydrolase antibody - Aminoterminal end (ab56146) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Anti-S adenosylhomocysteine hydrolase antibody - Aminoterminal end (ab56146) at 1 µg/ml + Human S adenosylhomocysteine hydrolase full length protein (ab99326) at 0.1 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Exposure time : 90 seconds