Anti-RPB2 antibody - ChIP Grade
| Name | Anti-RPB2 antibody - ChIP Grade |
|---|---|
| Supplier | Abcam |
| Catalog | ab10338 |
| Prices | $392.00 |
| Sizes | 50 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IP ICC/IF ICC/IF ChIP |
| Species Reactivities | Human, Arabidopsis thaliana, Mouse, Rat, Rabbit, Horse, Chicken, Guinea Pig, Bovine, Dog, Turkey, Pig, Xenopus, C. elegans, Chimpanzee, Drosophila, Monkey, Ape, Orangutan, Xenopus |
| Antigen | Immunogen was a synthetic peptide, which represented a portion of human DNA directed RNA polymerase II 140 kDa subunit encoded within exon 11 (LocusLink ID 5431) |
| Description | Rabbit Polyclonal |
| Gene | POLR2B |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab10338 stained DU145 cells. The cells were 4% paraformaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab10338, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
MED18 interaction with distinct transcription factors regulates multiple plant - MED18 interaction with distinct transcription factors regulates multiple plant
Lai Z, Schluttenhofer CM, Bhide K, Shreve J, Thimmapuram J, Lee SY, Yun DJ, Mengiste T. Nat Commun. 2014;5:3064.
POWERDRESS and diversified expression of the MIR172 gene family bolster the - POWERDRESS and diversified expression of the MIR172 gene family bolster the
Yumul RE, Kim YJ, Liu X, Wang R, Ding J, Xiao L, Chen X. PLoS Genet. 2013;9(1):e1003218.
The role of Mediator in small and long noncoding RNA production in Arabidopsis - The role of Mediator in small and long noncoding RNA production in Arabidopsis
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Intergenic transcription by RNA polymerase II coordinates Pol IV and Pol V in - Intergenic transcription by RNA polymerase II coordinates Pol IV and Pol V in
Zheng B, Wang Z, Li S, Yu B, Liu JY, Chen X. Genes Dev. 2009 Dec 15;23(24):2850-60.