![Western blot analysis on immunoprecipitation of 293 cell lysate labeling RNF20 using ab181104 at a 1/50 dilution and Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at a 1/1000 dilution and 5% NFDM/TBST blocking peptide.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/23888_ab181104-213586-ab181104-4.jpg)
Western blot analysis on immunoprecipitation of 293 cell lysate labeling RNF20 using ab181104 at a 1/50 dilution and Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at a 1/1000 dilution and 5% NFDM/TBST blocking peptide.
![Flow cytometry analysis of 2% paraformaldehyde fixed Jurkat cells labeling RNF20 with ab181104 at 1/290 dilution (red) or a Rabbit monoclonal IgG Isotype control (green), followed by Goat anti rabbit IgG (FITC) at a 1/150 dilution.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/23889_ab181104-213585-ab181104-3.jpg)
Flow cytometry analysis of 2% paraformaldehyde fixed Jurkat cells labeling RNF20 with ab181104 at 1/290 dilution (red) or a Rabbit monoclonal IgG Isotype control (green), followed by Goat anti rabbit IgG (FITC) at a 1/150 dilution.
![Immonofluorescence analysis of 4% paraformaldehyde fixed MCF7 cells, labeling RNF20 using ab181104 at a 1/250 dilution (green) followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody at a 1/2000 dilution and counterstained with Dapi (blue).](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/23890_ab181104-213584-ab181104-2.jpg)
Immonofluorescence analysis of 4% paraformaldehyde fixed MCF7 cells, labeling RNF20 using ab181104 at a 1/250 dilution (green) followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody at a 1/2000 dilution and counterstained with Dapi (blue).
![All lanes : Anti-RNF20 antibody [EPR13562(B)] (ab181104) at 1/5000 dilutionLane 1 : Jurkat cell lysateLane 2 : 293 cell lysateLane 3 : MCF7 cell lysateLane 4 : HeLa cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/23891_ab181104-213583-ab181104.jpg)
All lanes : Anti-RNF20 antibody [EPR13562(B)] (ab181104) at 1/5000 dilutionLane 1 : Jurkat cell lysateLane 2 : 293 cell lysateLane 3 : MCF7 cell lysateLane 4 : HeLa cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution