Immunohistochemical analysis of paraffin embedded Mouse colon tissue labeling RNA Helicase A with ab183731 at a 1/100 dilution. Prediluted HRP conjugated Rabbit IgG secondary used. Counterstained with Hematoxylin.
Flow cytometry analysis of HeLa cells using ab183731 at a 1/10 dilution (red) or a Rabbit monoclonal IgG (negative) (green). Goat anti rabbit IgG (FITC) secondary used at a 1/150 dilution.
Immunocytochemistry analysis of HeLa cells (fixative -20℃ Acetone) labeling RNA Helicase A with ab183731 at a 1/250 dilution.Goat anti rabbit IgG (Alexa Fluor® 488) secondary used at a 1/200 diution.
Immunohistochemical analysis of paraffin embedded Human lung adenocarcinoma tissue labeling RNA Helicase A with ab183731 at a 1/100 dilution. Prediluted HRP conjugated Rabbit IgG secondary used. Counterstained with Hematoxylin.
All lanes : Anti-RNA Helicase A antibody [EPR13521] (ab183731) at 1/50000 dilutionLane 1 : HeLa lysateLane 2 : Jurkat lysateLane 3 : 293 lysateLane 4 : BxPC-3 lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution