All lanes : Anti-RNA Helicase A antibody [EPR13520(B)] - N-terminal (ab180189) at 1/1000 dilutionLane 1 : BxPC-3 cell lysateLane 2 : Jurkat cell lysateLane 3 : HeLa cell lysateLane 4 : 293T cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labeled goat anti-rabbit at 1/2000 dilution
Immunofluorescent analysis of 293T cells labeling RNA Helicase A with ab180189 at 1/100.
Flow Cytometrical analysis of permeabilized Jurkat cells labeling RNA Helicase A with ab180189 at 1/10 (red) or a rabbit IgG negative (green).