All lanes : Anti-Ribonuclease Inhibitor antibody (ab77448) at 1 µg/mlLane 1 : Ribonuclease Inhibitor transfected 293T cell lysateLane 2 : Non-transfected 293T cell lysateLysates/proteins at 25 µg per lane.SecondaryGoat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution
Anti-Ribonuclease Inhibitor antibody (ab77448) at 1 µg/ml + Recombinant tagged Human Ribonuclease Inhibitor protein at 0.2 µgSecondaryGoat anti-mouse IgG (H&L)-HRP conjugate at 1/5000 dilution
![Overlay histogram showing A431 cells stained with ab77448 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab77448, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/23084_Ribonuclease-Inhibitor-Primary-antibodies-ab77448-1.jpg)
Overlay histogram showing A431 cells stained with ab77448 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab77448, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.